Fig. 1: Generation of MAPT−/− iPSCs.
From: Tau depletion in human neurons mitigates Aβ-driven toxicity
A Illustrations of gene editing strategies showing the positions of gRNA(s) in Exon 1 and 4 DNA loci. The intended double-stranded break in Exon 1 is indicated by an arrow, whereas the intended 25-bp deletion is indicated between the gRNA pair used to target Exon 4. B Sequencing results of edited MAPT loci, including all combinations of alleles in the Exon 1 isogenic panel. For the Exon 4 isogenic panel, both MAPT+/− and MAPT−/− lines harbour the 25-bp deletion at the same site. C Schematic of the cortical neuron differentiation protocol used throughout this study – iPSC lines were first differentiated concurrently to NPCs, before they were subject to lentiviral transduction for Ngn2 expression (“NPC-Ngn2 protocol”) for maturation either in co-culture with primary rat cortical astrocytes or in monoculture on coated surface.
