Fig. 4: Aβ-driven retrograde impairment of axonal transport of mitochondria is absent in MAPT−/− iPSC-derived cortical neurons.
From: Tau depletion in human neurons mitigates Aβ-driven toxicity
A Schematic of the experiments designed to measure axonal transport of mitochondria using microfluidic chambers. B Quantification of ratio of motile mitochondria (motile to stationary) in Day 70–95 iPSC-derived cortical neurons from the Exon 4 isogenic panel with or without directionality over 150 s of live imaging. The neurons were treated with either 2 μM scrambled Aβ1–42 or Aβ1–42 oligomers for 1 h before imaging. Mean ± SEM. n = 8 (MAPT + /+ scrambled Aβ1–42), 13 (MAPT + / + Aβ1–42), 6–9 (MAPT−/− scrambled Aβ1–42) and 12–14 (MAPT−/− Aβ1–42) microfluidic chambers measured across five independent neuronal differentiation repeats. Two-tailed Mann-Whitney test was used for statistical analysis.
