Fig. 2: Inflammation, cytokine release and phagocytosis in iMGLs.
From: Genetic contribution to microglial activation in schizophrenia
a Gene expression of inflammatory related HLA-DRA, TSPO and IL1β based on RNA sequencing. b Representative image after 4-h migration assay. Cells were masked with magenta for analysis. Scale bar 800 µm. c The number of migrated cells normalized to unstimulated condition for each group. Performed Kruskal–Wallis test (H(2) = 2.371, p = 0.3305; H(2) = 0.7829, p = 0.6927). d Release of IL6 (CTRL: χ2(3) = 10.33, p = 0.0030; ST: χ2(3) = 1.114, p = 0.8307; HT: χ2(3) = 5.864, p = 0.1132), IL8 (CTRL: χ2(3) = 8.400, p = 0.0190; ST: χ2(3) = 7.500, p = 0.0517; HT: χ2(3) = 7.800, p = 0.0443) and MCP1 (CTRL: χ2(3) = 5.100, p = 0.1897; ST: χ2(3) = 11.10, p = 0.0009; HT: χ2(3) = 9.72, p = 0.0120) cytokines after 24 h treatment with either 20 ng/ml IL1β, 20 ng/ml TNFα or 20 ng/ml IFNγ. Friedman test and Mann–Whitney test. e Gene expression of TLR2 and TLR4. f Representative image from phagocytosis after 6 h (Scale bar 100 µm) and (g) phagocytosis of pHrodo-zymosan bioparticles. h Phagocytosis between the groups after 6 h (H(2) = 0.7800, p = 0.7027). Kruskal–Wallis test. n = 4–5 cell lines.
