Fig. 2: mGlu5 in SST+ neurons modulate network activity in a sex- and region-specific manner.
From: Loss of mGlu5 receptors in somatostatin-expressing neurons alters negative emotional states
a Example images of mGlu5 (magenta), SST (turquoise), and PV (yellow) immunoreactivity in O-LM INs from sections of a WT (left panels, scale bar 5 μm, thickness of the z-stack 2.21 μm) and a SSTCre-Grm5−/− mouse brain (right panels, scale bar 5 μm, thickness of the z-stack: 3.45 μm). The absence of mGlu5 labeling along the plasma membrane (outlined in white in the merged upper panels) of SSTCre-Grm5−/− O-LM INs confirms the recombination and, in turn deletion of Grm5 from SST+ neurons. b Image depicting the MEA stimulating and recording electrodes position within a hippocampal mouse slice. Red circles represent stimulating electrodes and blue ones recording electrodes. c Upon TBS, slices from SSTCre-Grm5−/− mice displayed increased LTP (fEPSP amplitude in %) compared to WT mice. Two-way ANOVA, Genotype F1,73 = 5.317, *p < 0.05. d The increased LTP in SSTCre-Grm5−/− compared to WT mice was more prominent in the late LTP phase (Early LTP: Mann–Whitney U = 514, p > 0.05; Late LTP: Mann–Whitney U = 558, *p < 0.05). e Fold change frequency ratios upon DHPG stimulation in the CA3 region of the HPC were larger in SSTCre-Grm5−/− compared to WT male mice. Mann–Whitney U = 20884, *p < 0.05. f Fold change frequency ratios upon DHPG stimulation in the CA1 region of the HPC were reduced in SSTCre-Grm5−/− compared to WT male mice. Mann–Whitney U = 24475, *p < 0.05. g Fold change frequency ratios upon DHPG stimulation in the mPFC were reduced in SSTCre-Grm5−/− compared to WT male mice. Mann-Whitney U = 49767, **p < 0.01. h, i Fold change frequency ratios upon DHPG stimulation in the CA3 (Mann–Whitney U = 28391, p > 0.05) (h) and CA1 (Mann–Whitney U = 11080, p > 0.05), i region of the HPC was similar in SSTCre-Grm5−/− and WT female mice. j Fold change frequency ratios upon DHPG stimulation in the mPFC were reduced in SSTCre-Grm5−/− compared to WT female mice. (Mann–Whitney U = 56486, *p < 0.05). Data are shown as mean ± SEM. *p < 0.05, **p < 0.01.
