Fig. 1: Generation of neurons from healthy twin sets and from twin sets that are discordant for schizophrenia.

A A table describing the cohort of patients and control individuals. The lines with the information of the affected patients are highlighted in pink and the unaffected twin siblings (depressive disorder) are highlighted in light blue. The green lines contain information about the healthy control twin sets. B A schematic of the reprogramming and differentiation and the experiments performed. C Upper row, left – Pluripotency markers were expressed in the reprogrammed iPSCs. Upper row, Right – Neural Progenitor cells (NPCs) expressed Nestin and PAX6–NPC-specific markers. Lower row – differentiated neurons expressed MAP2 and approximately 80% expressed Prox1, a marker that is specific for the DG granule neurons in neurons derived from the 3 groups of affected twin siblings, unaffected co-twin siblings, and healthy twin sets. Scale bars are 50 μm.