Fig. 1: Schematic diagram showing the experimental design and analysis pipeline used in this study.
From: Genetic regulation of human brain proteome reveals proteins implicated in psychiatric disorders
A Postmortem brain samples from a human cohort with 268 participants were used, including 198 normal individuals (CTR), 45 patients with schizophrenia (SCZ), and 25 patients with bipolar (BP). B Deep brain proteome was profiled by 11-plex TMT-based proteomics, followed by extensive quality control and data analysis. Brain proteomic data and comparable genotype data were prepared for subsequent linkage analysis. C Genome-wide association analysis to identify genetic regulations of protein expression and gene expression. D Co-localization analysis to investigate the same variant underlying cis-eQTLs and cis-pQTLs. E Mediation analysis to identify transcript-dependent and -independent regulations and causality analysis to link eGenes and pGenes to SCZ GWAS loci. F Prioritization of proteins for SCZ GWAS loci.
