Fig. 6: Knockdown of A1 receptor blocked 40 Hz light flicker’s beneficial effects on social behavior and excitatory transmission. | Molecular Psychiatry

Fig. 6: Knockdown of A1 receptor blocked 40 Hz light flicker’s beneficial effects on social behavior and excitatory transmission.

From: Adenosine mediates the amelioration of social novelty deficits during rhythmic light treatment of 16p11.2 deletion female mice

Fig. 6

a Representative images of A1R immunostaining in two groups, scale bar: 40 μm. b Quantification of A1R intensity in GFP+ cells (16p+control virus+40Hz-14d: n = 120 cells from 4 mice, 16p + A1 receptor shRNA virus+40Hz-14d: n = 150 cells from 5 mice). c The trajectory chart of mice in the open field in three groups. Dashed box: center area. d The distance traveled in the open field. e Time in center in open field (16p: n = 7, 16p+control virus+40Hz-14d: n = 7, 16p + A1 receptor shRNA virus+40Hz-14d: n = 6). f The trajectory chart of mice in the three-chamber test. S1: familiar mouse, S2: novel mouse. g Sociability index in the three-chamber test. h Social novelty preference index in three-chamber test (16p: n = 7, 16p+control virus+40Hz-14d: n = 7, 16p + A1 receptor shRNA virus+40H z-14d: n = 6). i Representative traces of sEPSC recorded in PFC in two groups. j Quantification of sEPSC frequency. k Quantification of sEPSC amplitude (16p+control virus+40Hz-14d: n = 23 cells from 5 mice, 16p + A1 receptor shRNA virus+40Hz-14d: n = 26 cells from 5 mice). l Representative traces of paired-pulse stimulation at the interval of 50 ms, 100 ms, and 200 ms in two groups, scale bar: 100 pA/50 ms. m Quantification of EPSC PPR (16p+control virus+40 Hz-14d: n = 15 cells from 5 mice, 16p + A1 receptor shRNA virus+40Hz-14d: n = 15 cells from 5 mice). n Representative images of VGLUT1 immunostaining in two groups. o Quantification of VGLUT1+ boutons surrounding cells (16p+control virus+40Hz-14d: n = 51 cells from 4 mice, 16p + A1 receptor shRNA virus+40Hz-14d: n = 38 cells from 3 mice), scale bar: 10 μm. p Representative images of Golgi staining in two groups, scale bar = 3 μm. q Quantification of dendritic spines (16p+control virus+40Hz-14d: 38 dendrites from 3 mice, 16p + A1 receptor shRNA virus+40Hz-14d: 34 dendrites from 3 mice). ns, not significant, *P < 0.05, ***P < 0.01, unpaired t test, Mann-Whitney U test, and two-way RM ANOVA with Sidak’s post hoc test.

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