Table 1 Proteins involved in neuropeptide release.
Proteins | Protein family | Neuropeptide, cell type and/or release measurement | Effects | References |
|---|---|---|---|---|
CAPS1 and 2 | Release site proteins and other regulators | Neurotrophin-3 release assessed with enzyme-linked immunoassay (ELISA) in cultured cerebellar neurons; BDNF-pHluorin, Semaphorin-3A-pHluorin, NPY-pHluorin, or NPY-mCherry release in cultured hippocampal neurons | Knockout of CAPS1 and/or CAPS2 impairs release to various degrees | |
Complexin1 and 2 | Release site proteins and other regulators | IGF-1 levels assessed with ELISA, Syt-10-pHluorin in cultured olfactory bulb neurons; BDNF-quantum dot and BDNF-EGFP release in cultured hippocampal neurons | Knockdown impairs release | |
Dynamin 1, 2, and 3 | Release site proteins and other regulators | NPY-pHluorin and BDNF-pHluorin in cultured hippocampal neurons | Knockout impairs release | [244] |
Munc13-1 and -2 | Release site proteins and other regulators | Semaphorin-3A-pHluorin and NPY-pHluorin in cultured hippocampal neurons | Knockout impairs release | [222] |
Munc18-1 | Release site proteins and other regulators | NPY-pHluorin, BDNF-pHluorin and NPY-mCherry release in cultured hippocampal neurons | Knockout strongly impairs release | [245] |
RAB3A, B, C, and D | Release site proteins and other regulators | NPY-pHluorin, BDNF-pHluorin and BDNF release monitored by ELISA in cultured hippocampal neurons | Knockout strongly impairs release | [227] |
RIM1 and 2 | Release site proteins and other regulators | NPY-pHluorin, BDNF-pHluorin and NPY-mCherry release in cultured hippocampal neurons | Knockout strongly impairs release | [227] |
SNAP-25 | SNARE proteins | BDNF-pHluorin in cultured cortical neurons; NPY-pHluorin in cultured hippocampal neurons | Knockdown or knockout strongly impair release | |
SNAP-47 | SNARE proteins | BDNF-pHluorin in cultured cortical neurons | Knockdown impairs release | |
Synaptobrevin-2/Vamp2 | SNARE proteins | BDNF-pHluorin in cultured cortical neurons | Knockdown strongly impairs release | [246] |
Synaptotagmin-1 | Synaptotagmins | NPY-pHluorin in cultured hippocampal neurons | Knockdown or knockout impairs release | [248] |
Synaptotagmin-4 | Synaptotagmins | BDNF levels assessed with ELISA in cultured hippocampal neurons | Knockout mildly increases release | [223] |
Synaptotagmin-6 | Synaptotagmins | BDNF-quantum dot and BDNF-EGFP release in cultured hippocampal neurons | Knockdown impairs BDNF-quantum dot release but not BDNF-EGFP release | [243] |
Synaptotagmin-7 | Synaptotagmins | NPY-pHluorin in cultured hippocampal neurons | Knockout impairs release | [248] |
Synaptotagmin-9 | Synaptotagmins | Substance P-pHluorin in cultured striatal neurons | Knockout impairs release | [249] |
Synaptotagmin-10 | Synaptotagmins | IGF-1 levels assessed with ELISA in cultured olfactory bulb neurons | Knockout impairs release | [228] |
Vti1a and b | SNARE proteins | NPY-pHluorin in cultured hippocampal neurons | Knockout strongly impairs release; effects are due to Golgi sorting function | [250] |
