Fig. 1: The ensemble of PSTN neurons activated by refeeding delays consumption of familiar and palatable foods but hastens water drinking. | Molecular Psychiatry

Fig. 1: The ensemble of PSTN neurons activated by refeeding delays consumption of familiar and palatable foods but hastens water drinking.

From: The parasubthalamic nucleus refeeding ensemble delays feeding initiation and hastens water drinking

Fig. 1

A Experimental design and representative image of mCherry fluorescence showing targeted recombination in the PSTN of a mouse injected with 4-OHT immediately prior to refeeding (scale bars: gray, 500 μm; white, 200 μm). B The number of PSTN mCherry-positive cells illustrates the extent of recombination across experimental conditions. Behavioral testing was performed 30 min after injection of the chemogenetic actuator CNO, and 24 h after food deprivation for chow (C) and Froot Loops (D), or 4 h after water deprivation for water (E) and sucrose (F). In each panel, the latency to initiate feeding/drinking is shown on the left and the amount of food/fluid consumed is shown on the right. Bars represent mean ± s.e.m. and individual values are overlaid. Data were analyzed using one-way ANOVA followed by Tukey’s posthoc comparisons when appropriate, *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001.

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