Fig. 2: PSTNTac1 neurons delay and reduce food and fluid consumption independently of caloric content, metabolic state, and novelty.
From: The parasubthalamic nucleus refeeding ensemble delays feeding initiation and hastens water drinking
A Experimental design and representative images of mCherry fluorescence in the PSTN illustrating expression of hM3Dq in PSTNTac1 neurons (scale bars: gray, 500 μm; white, 200 μm). Behavioral testing was performed 30 min after injection of the chemogenetic actuator CNO, and 24 h after food deprivation for chow (B) and Froot Loops (C, D, no food deprivation but same circadian timepoint in E), or 4 h after water deprivation for water (F) and sucrose (G, H). Testing was performed upon first-time access to Froot Loops and sucrose (C, G), and again following habituation (D, H). Froot Loops consumption was also tested after habituation to scheduled access with concurrent ad libitum access to chow (E). In each panel, the latency to initiate feeding/drinking is shown on the left and the amount of food/fluid consumed is shown on the right. Bars represent mean ± s.e.m. and individual values are overlaid. Data were analyzed using one-way ANOVA followed by Dunnett’s posthoc comparisons to mCherry controls, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
