Fig. 4: CeA- but not BNST- nor PVT-projecting PSTN neurons mediate refeeding delay.
From: The parasubthalamic nucleus refeeding ensemble delays feeding initiation and hastens water drinking
A Experimental design and representative images of GFP fluorescence illustrating Cre expression and mCherry fluorescence illustrating hM3Dq expression in the PSTN (scale bars: gray, 500 μm; white, 200 μm). Cre was expressed from a retrograde vector injected either in the CeA (B, C), BNST (D, E), or PVT (F, G). Behavioral testing was performed 30 min after injection of the chemogenetic actuators CNO (B, D, F) or SalB (C, E, G), or their respective vehicles, 24 h after food deprivation. Chow was presented either in the home cage (B, D, F) or in a novel arena (C, E, G). In each panel, the latency to initiate feeding/drinking is shown on the left and the amount of food/fluid consumed is shown on the right. Bars represent mean ± s.e.m. and individual values are overlaid. Data were analyzed using paired t tests, *p < 0.05; **p < 0.01; ***p < 0.001.
