Fig. 2: Deletion of Nrp2 at an embryonic stage results in reduced numbers of somatostatin (SOM) and neuropeptide Y (NPY) expressing neurons in the CA1 region of the hippocampus.

Immuno-labeled of littermate control A, C, and iCKO B, D brain sections, respectively, with anti-SOM (green) and DAPI (blue). High magnification images C, D of area in red boxes in A, B, respectively. Scale bars: 100 μm. E Quantification of total mean SOM+ hippocampal cells. Error bars are ± SEM; unpaired t-test: *p = 0.0426. Significantly fewer number of SOM+ neurons found in hippocampus of iCKO compared to control mice. F Quantification of the average number of SOM+ cells by hippocampal region. Significantly lower SOM+ cell density found in CA1 region of iCKO mice compared to littermate controls; ***p = 0.0004. n = 4 animals/genotype. G–J Immuno-labeled of littermate control G, I and iCKO H, J brain sections, respectively, with anti-NPY (yellow) and DAPI (blue). High magnification images I, J of area in green boxes in G, H respectively. Scale bars: 100 μm. K Quantification of total mean NPY+ hippocampal cells. Error bars are ± SEM; two-way ANOVA, post-hoc Bonferroni for multiple comparisons: ***p = 0.0003. Significantly fewer number of NPY+ neurons found in hippocampus of iCKO compared to control mice. L Quantification of the average number of NPY+ cells by hippocampal region. Significantly lower NPY+ cell density found in CA1 region of iCKO mice compared to littermate controls; ****p < 0.0001. n = 4 animals/genotype.