Fig. 6: The regulation of Gem by OXT is polarized depending on cell type.
From: OXTR-mediated signaling in astrocytes contributes to anxiolysis
Effect of OXT (100 nM, 180 min) on protein levels of Gem (a; t13 = 3.700, **p = 0.003), ROCK1 (b; t14 = 3.744, **p = 0.002), pMYPT/Thr696 (c; t12 = 3.244, **p = 0.007) and Sp1 (d; t12 = 8.751, ***p < 0.001) in H32 neurons assessed by immunoblotting. Representative bands are shown below. e Effects of OXT on neurite length of H32 cells pre-treated with either Veh or the ROCK-inhibitor y-27632 (interaction: F1,1884 = 7.396, p = 0.007, ***p < 0.001 Veh/Veh vs. Veh/OXT). f, g Representative images of hippocampal astrocytes (GFAP; green) co-stained with Gem (red) and DAPI (blue) in rats that received either Veh or OXT icv. Quantification of above threshold (Gem+) cells was defined as single cells marked by DAPI/GFAP (Gem+/GFAP+; t10 = 2.771, *p = 0.020) or DAPI/absence of GFAP (Gem+/GFAP-; t10 = 2.606, *p = 0.029) staining displaying at least one maximum of above threshold Gem fluorescence. Data represent mean relative or absolute values +/−SEM.
