Fig. 3: Multiple predicted GSK3 and AKT phosphosites in βIV spectrin.

A Scheme of phosphosite prediction pipeline using βIV spectrin sequence and eukaryotic linear motif phosphosite predictor. B Predicted peptide sequence logos for GSK3 (left) and AKT (right). Amino acids are colored based on their chemical composition. Red - acidic, blue - basic, black - hydrophobic, purple – neutral, and green - polar. C βIV spectrin sequences predicted to be phosphorylated by either GSK3 or AKT. D Additional in silico prediction of phosphosites using the Phosphosite Plus Kinase Library showing likelihood of phosphorylation by GSK (blue) or AKT (green). Darker color indicates greater likelihood of phosphorylation of specific residues by GKS3 or AKT (ranked 1–100, 1 = highest likelihood). Gray squares indicate no phosphorylation sites at specific residues E. Matching key for ranked sites in D.