Fig. 6: Accurate SCZ classification with Random Forest classifier of βIV spectrin image features.

A Zoom to AIS of HC and SCZ neurons treated with vehicle, GSK3 or AKT inhibitor showing MAP2 (blue) and accumulation of βIV spectrin (red) at the AIS. White arrows indicate beginning and end of AIS ROI and the scale bar is 2 μm. B AIS fluorescence intensity signal of βIV spectrin. C Classification accuracy based on sorting signals between HC and SCZ groups for DMSO, GSK3 inhibitor, and AKT inhibitor-treated neurons. D Classification accuracy based on sorting signals between DMSO and GSK3 inhibitor-treated cells for HC and SCZ neurons, respectively. E Classification accuracy based on sorting signals between DMSO and AKT inhibitor-treated cells for HC and SCZ neurons, respectively. F Table of classification accuracy of all groups. The highest sorting accuracy was from SCZ neurons treated with AKT inhibitor.