Fig. 5: Normalization of abnormal H3K4 methylation in the adult hippocampus through pharmacological inhibition of LSD1. | Molecular Psychiatry

Fig. 5: Normalization of abnormal H3K4 methylation in the adult hippocampus through pharmacological inhibition of LSD1.

From: Recapitulation and reversal of neuropsychiatric phenotypes in a mouse model of human endogenous retrovirus type W expression

Fig. 5

Male transgenic CAGHERV-Wenv mice (TG) and wild-type (WT) littermates were treated with the LSD1 inhibitor, ORY-1001, or corresponding vehicle (VEH). The scatter plots (with overlaid means ± s.e.m.) depict the Western blot analysis of H3K4 mono-methylation (H3K4me1), di-methylation (H3K4me2) and tri-methylation (H3K4me3), normalized to H3 housekeeping control, in the hippocampus of WT and TG mice. *p < 0.05 and **p < 0.01, based on Tukey’s post-hoc test following a significant 2-way interaction between genotype and treatment in ANOVA of H3K4me2 (F(1,34) = 4.89, p < 0.05) and H3K4me3 (F(1,34) = 4.18, p < 0.05). #p = 0.064, reflecting the main effect of treatment at statistical trend level (F(1,34) = 3.49). n(WT/VEH) = 10 mice, n(TG/VEH) = 10 mice, n(WT/ORY-1001) = 9 mice, and n(TG/ORY-1001) = 9 mice.

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