Fig. 6: APOE deficiency halted conversion of homeostatic microglia in an ATN model. | Molecular Psychiatry

Fig. 6: APOE deficiency halted conversion of homeostatic microglia in an ATN model.

From: APOE deficiency inhibits amyloid-facilitated (A) tau pathology (T) and neurodegeneration (N), halting progressive ATN pathology in a preclinical model

Fig. 6

A Schematic overview representing the single cell RNA-sequencing setup used for WT, F +T + ApoE WT and F +T + ApoE KO brain isolation (n = 6 per genotype), single cell isolation, sequencing and analysis. B UMAP-projection of cells analysed (WT = 4838, F +T + ApoE WT = 2671, F +T + ApoE KO = 3830). nc monocytes: nonclassical monocytes. C Dot plot visualizing expression of key marker genes for each of the clusters that were identified in B, D UMAP projection of the microglia cluster in their respective experimental groups showing different microglial phenotypes: homeostatic microglia (hMg, yellow), reactive microglia (rMg, orange), disease-associated microglia (DAM, red) and interferon-signalling microglia (IFN-sign Mg, blue). Only coloured cells belong to the specified condition, cells belonging to other conditions are depicted in grey. The pie charts represent the percentages of the 4 distinct microglia subsets, within total microglia, per condition. E Dot plot representing the expression of signature genes for the different microglial genotypes.

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