Fig. 1: CeA D2Rs regulate compulsive-like eating. | Molecular Psychiatry

Fig. 1: CeA D2Rs regulate compulsive-like eating.

From: Dopamine D2 receptor modulation of insulin receptor signaling in the central amygdala: implications for compulsive-like eating behavior

Fig. 1

A Experimental scheme for food self-acquisition task. FR: fixed ratio, FS: foot shock (scrambled shock, 0.3 mA, 0.5 s), PF: palatable food, A: active lever, I: inactive lever. B Number of active lever presses per hour by WT and Drd2−/− mice across days. Two-way repeated measures ANOVA, group×session interaction: F3,78 = 4.77, **p = 0.0042. C Number of active lever presses per hour from FR1 to FR3 sessions. One-way ANOVA followed by Bonferroni post hoc tests, ###p < 0.001 vs. FR1, ***p < 0.001 vs. WT. D Number of active lever presses per hour in the last 3 days of FR3 + FS sessions. Unpaired Student’s t-test, two-tailed, **p = 0.0060. E Number of active lever presses per hour in FS only sessions. WT n = 12, Drd2−/− n = 16. F Schematic diagram of AAV-GFP or AAV-Cre-GFP injection into the CeA of Drd2flox/fox mice. G Left: Representative images of AAV-GFP and AAV-Cre-GFP virus expression in the CeA at low magnification. Virus expression was observed in three sections per mouse used for experiments. GFP control n = 3, Cre-GFP n = 4. Right: Drd2 mRNA expression in the CeA of GFP and Cre-GFP virus-injected mice as detected by FISH. Red: Drd2, green: GFP or Cre-GFP, blue: DAPI. Scale bar: low magnification, 100 μm, high magnification, 20 μm. H Quantitative analysis of Drd2 mRNA expression in the CeA. Proportion of Drd2 mRNA in GFP and Cre- GFP virus-injected mice. Unpaired Student’s t-test, two-tailed, **p = 0.0054. GFP control n = 3, Cre-GFP n = 4. I Number of active lever presses per hour by GFP and Cre-GFP virus-injected mice across days. Two-way repeated measures ANOVA, group×session interaction: F5,75 = 5.63, ***p = 0.0002. J Number of active lever presses per hour from FR1 to FR3 sessions. One-way ANOVA followed by Bonferroni post hoc tests, ###p < 0.001 vs. FR1. K Number of active lever presses per hour in the last 3 days of FR3 + FS sessions. Unpaired Student’s t-test, two- tailed, **p = 0.0033. L Number of active lever presses per hour in FS only sessions. GFP n = 8, Cre-GFP n = 6. All values in the data represent mean ± SEM.

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