Fig. 3: Selective loss of InsRs in CeA D2R-expressing neurons results in stronger compulsive-like perseverance of PF-seeking behavior. | Molecular Psychiatry

Fig. 3: Selective loss of InsRs in CeA D2R-expressing neurons results in stronger compulsive-like perseverance of PF-seeking behavior.

From: Dopamine D2 receptor modulation of insulin receptor signaling in the central amygdala: implications for compulsive-like eating behavior

Fig. 3

A Schematic diagram of AAV-DIO-eYFP or AAV-DIO-DSE-mCherry-PSE-shInsR injection into the CeA of Drd2-Cre mice. B Representative InsR expression in the CeA of CeA-D2ReYFP control and CeA-D2RshInsR mice. Virus expression was observed in three sections per mouse used for experiments. CeA-D2ReYFP n = 3, CeA-D2RshInsR n = 3. Red: eYFP or shInsR, green: InsR, blue: DAPI. Scale bar: low magnification, 100 μm, high magnification, 20 μm. C Quantitative analysis of InsR expression in the CeA of CeA-D2ReYFP control and CeA-D2RshInsR mice. CeA-D2ReYFP n = 3, CeA-D2RshInsR n = 3. Unpaired student’s t-test, two-tailed, *p = 0.0344. D Number of active lever presses per hour by CeA-D2ReYFP and CeA-D2RshInsR mice across days. CeA-D2ReYFP n = 3, CeA-D2RshInsR n = 4. E Number of active lever presses per hour in FR1 to FR3 sessions. One-way ANOVA followed by Bonferroni post hoc tests, #p < 0.05, ###p < 0.001 vs. FR1. CeA-D2ReYFP n = 3, CeA-D2RshInsR n = 4. F Number of active lever presses per hour in the first 3 days of FR3 + FS sessions. Unpaired Student’s t-test, two-tailed, **p = 0.0061. CeA-D2ReYFP n = 3, CeA- D2RshInsR n = 4. G Number of active lever presses per hour in FS only sessions. CeA-D2ReYFP n = 3, CeA-D2RshInsR n = 4. All values in the data represent mean ± SEM.

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