Fig. 5: PF and Insulin modulate CeA D2R neuronal activity. | Molecular Psychiatry

Fig. 5: PF and Insulin modulate CeA D2R neuronal activity.

From: Dopamine D2 receptor modulation of insulin receptor signaling in the central amygdala: implications for compulsive-like eating behavior

Fig. 5

A Experimental scheme of light/dark box test and fiber photometry with drug infusion. B Normalized z-score during PF consumption after saline infusion. C Average z-score during PF consumption after saline infusion. Saline: Drd2-Cre n = 7. Paired student’s t-test, two-tailed, **p = 0.0095. D Normalized z-score during PF consumption after quinpirole (1 µg), insulin (5 mU), or quinpirole+insulin infusion. E-G Average z-score during PF consumption after drug infusion. E Quinpirole: Drd2-Cre n = 10. Paired student’s t-test, two-tailed, *p = 0.0153. F Insulin: Drd2-Cre n = 10. Paired student’s t-test, two-tailed, **p = 0.0045. G Quin+Ins: Drd2-Cre n = 9. Paired student’s t-test, two-tailed, p = 0.3200. H Normalized z-score during PF consumption after sulpiride (1 µg), sulpiride+quinpirole, or sulpiride+quinpirole+insulin infusion. I-K Average z-score on pre (-2s to 0 s) and post (0 s to +10 s) during PF consumption after drug infusion. I Sulpiride: Drd2-Cre n = 12. Paired student’s t-test, two-tailed, **p = 0.0021. J Sulp+Quin: Drd2-Cre n = 13. Paired student’s t-test, two-tailed, *p = 0.0442. K Sulp+Quin+Ins: Drd2-Cre n = 11. Paired student’s t-test, two-tailed, p = 0.8766. L PF consumption for 10 min. in drug-infused mice. Saline, Quinpirole, Insulin, Quin+Ins Drd2-Cre n = 13. Repeated measure One-way ANOVA, Bonferroni posthoc tests **p = 0.0048, #p < 0.05 vs. saline. Sulpiride, Sulp+Quin, and Sulp+Quin+Ins Drd2- Cre n = 13. Repeated measure One-way ANOVA, Bonferroni posthoc tests, ***p = 0.0002, †††p < 0.001 vs. saline, **p < 0.01, ***p < 0.001 vs. group. Solid line: average z-score of food consumption. Shaded line: standard error of the mean. All values in the data represent mean ± SEM.

Back to article page