Fig. 5: Calcium imaging indicates a hypoactive phenotype in ADHD patient CNs.
From: hiPSC-derived cortical neurons from ADHD individuals reveal dysregulated glutamatergic development
A Representative calcium imaging trace of one HC neuron depicting peak amplitude (red circle). B Mean peak amplitude was measured at 8, 10 and 12 weeks during neuronal maturation in HC (n = 4; except for 8-week measurement, n = 3) and ADHD patients (n = 4; except for 8-week measurement, n = 3), and a trend for decreased mean peak amplitude in ADHD patient CNs was observed at all time points. C The same trend was observed when assessing maximum peak amplitude in the same cells. D Representative calcium imaging trace of one HC neuron depicting calcium peaks (red circle). E Mean number of calcium peaks over time was quantified at 10 and 12 weeks in HC (n = 4) and ADHD patient (n = 4) CNs. A marginally significant decrease in calcium peak rate was observed at 10 weeks, and a significant decrease at 12 weeks. F Maximum calcium peak rate reflected these results, with significant decreases observed at both 10 and 12 weeks. Data was analysed using independent t-tests. CNs cortical neurons, HC healthy controls. *p < 0.05.
