Fig. 5: Single-nuclei RNA sequencing (snRNA-seq) for exploring EPO and EPOR expression.

A UMAP clustering of nuclei from the mature CA1 and newly formed clusters are labelled and encircled (top). Feature plot based on the UMAP plot showing the single nuclei expression of EPO, Emx1 and EPOR in pyramidal neurons. B Volcano plot showing genes that are differentially expressed between EPO-positive and EPO-negative pyramidal neurons. The horizontal dashed line indicates -Log₁₀P = 1 (FDR corrected two-way Fisher’s exact test). Differentially expressed genes are colored blue (down-regulated) and red (up-regulated). The top differentially expressed genes are shown which contribute to the gene ontology in the next figure subset. C Enriched pathways, and gene ontologies, correspond to phenotypes and transcription factor target genes among genes that are differentially expressed (colored in the previous plot). Data is shown as an enrichment score (observed/expected), with -log10 hypergeometric P-value < 0.05. D EPOR expression in the analyzed pyramidal cell types is upregulated in EPO-negative (purple) when compared with EPO-positive (grey) nuclei. Every dot shown on the plot represents a nucleus from snRNA-seq data. Transcript abundance in snRNA-Seq data is shown as log-transformed expression of EPOR normalized using the transcriptome. E The stacked bar plot shows the percentage of nuclei expressing EPOR in 2 distinct groups of nuclei from EPO-positive and EPO-negative pyramidal neurons.