Fig. 2: The R702W substitution affects relative levels of SorCS2 processed forms and glycosylation pattern. | Molecular Psychiatry

Fig. 2: The R702W substitution affects relative levels of SorCS2 processed forms and glycosylation pattern.

From: A low frequency damaging SORCS2 variant identified in a family with ADHD compromises receptor stability and quenches activity

Fig. 2

A and C representative western blot (n = 5) showing reduced levels of the one and two chain SorCS2 R702W in HEK293T and SH-SY5Y cells. B and D quantification of A and C. E levels of one- and two-chain SorCS2 R702W are reduced in transfected human iPSC-derived neurons (n = 3, experiments performed in technical duplicates). Of note, these cells express endogenous SorCS2 as seen with the mock transfected cells. F quantification of E shown with endogenous level of SorCS2 subtracted. G western blot showing that all N-linked oligosaccharides of the proform of SorCS2 R702W can be fully removed with endo H treatment suggesting that the proform of SorCS2 R702W contains mainly high-mannose N-linked oligosaccharides (n = 3).

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