Fig. 3: The R702W substitution causes aberrant subcellular localization of SorCS2. | Molecular Psychiatry

Fig. 3: The R702W substitution causes aberrant subcellular localization of SorCS2.

From: A low frequency damaging SORCS2 variant identified in a family with ADHD compromises receptor stability and quenches activity

Fig. 3

A, C and E coimmunostaining of SorCS2 variants and calnexin (A), TGN46 (C), and EEA1 (E) in transfected iPSC-derived neurons. Hoechst staining is shown in blue. B D, F quantification of A, C, and E shown as Pearson’s correlation coefficient for SorCS2 variants and respective subcellular markers. Results are shown as mean ± SEM, n = 69 (SorCS2 WT, calnexin), n = 49 (SorCS2 R702W, calnexin), n = 10 (SorCS2 WT, TGN46), n = 13 (SorCS2 R702W, TGN46), n = 22 (SorCS2 WT, EEA1), n = 27 (SorCS2 R702W, EEA1), statistical analysis by unpaired two tailed Student’s t test, IF, immunofluorescence. Scale bar is 5 μm.

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