Fig. 6: The R702W variant forms dimers and quenches SorCS2 WT activity. | Molecular Psychiatry

Fig. 6: The R702W variant forms dimers and quenches SorCS2 WT activity.

From: A low frequency damaging SORCS2 variant identified in a family with ADHD compromises receptor stability and quenches activity

Fig. 6

A western blot showing homodimerization of both WT and R702W SorCS2 in transfected HEK293T cells after cross-linking with DSP (n = 3). B R702W SorCS2 can be co-immunoprecipitated with WT SorCS2 tailless in HEK293T cells as assessed with an antibody recognizing SorCS2 intracellular domain (ICD) (n = 3). C western blot showing that SorCS3 co-immunoprecipitates equally well with WT and R702W SorCS2 in transfected HEK293T cells (n = 3). D Sorcs2 knockout hippocampal neurons were transfected with the indicated constructs and GFP and neuronal branching assessed in the presence or absence of 1 nM BDNF for 72 h. Scale bar is 100 μm. E quantification of D. Values are shown as mean ± SEM (n = 5). Statistical analysis by unpaired two tailed Student’s t test.

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