Fig. 3: Hippocampal SGK1 determines stress vulnerability in mice. | Molecular Psychiatry

Fig. 3: Hippocampal SGK1 determines stress vulnerability in mice.

From: Hippocampal SGK1 promotes vulnerability to depression: the role of early life adversity, stress, and genetic risk

Fig. 3

A Timeline of LBN experiment. B SGK1 mRNA is increased in the DG of LBN-reared mice. C Timeline of CORT experiment. D SGK1 mRNA is increased in the DG of CORT-treated mice. E Timeline of CSDS experiment and subpopulations of susceptible and resilient mice following CSDS. F SGK1 expression is increased in the DG of susceptible, but not resilient mice. G SGK1 expression in the mouse DG negatively correlates with SI ratio. H Cre-virus injections into the DG of SGK1flox/flox mice. I SGK1 mRNA expression is reduced by ~85% at 5 weeks after Cre-virus injections. J Timeline of surgeries and behavior testing for SGK1 knockdown experiments. K mCherry expression indicating Cre virus expression in the DG of SGK1flox/flox mice 5 weeks after injection. Scale bars, 200μm. L DG-specific SGK1 knockdown does not affect interaction with an empty enclosure. M SGK1 knockdown prevents CSDS effects on social avoidance in the SI test, and N reduces the fraction of susceptible mice based on the SI ratio. DG – Dentate Gyrus; CORT – Corticosterone; CSDS – Chronic Social Defeat Stress; SI – Social Interaction. *p < 0.05; **p < 0.01.

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