Fig. 2: Weighted miRNAs co-expression network analysis.

A Dendrogram depicting results of hierarchical clustering, bottom color strip indicates distribution of the final 12 modules. B Boxplots portraying distribution of average log₂FC values (calculated as mean of log2FC values from individual studies included in meta-analysis) of miRNAs in individual modules. Bottom table shows number of miRNAs in each module. Modules E, F, and K (depicted in bold) were characterized by the most significantly changed log2FC values (Q1–Q3 ≠ 0) and contained the highest proportion of miRNAs significantly altered in AD as revealed by the meta-analysis. C Barplot showing proportion of miRNAs changed in AD patients based on meta-analysis in individual modules. E, F and K modules with most significantly increased proportions of changed miRNAs in AD patients are emphasized in bold. Modules C, G, H and L exhibit least miRNA changes. D Heatmaps showing presence of miRNAs from modules E, F and K in individual tissues (85[100%], 162 [95%] and 75 [93%]) of miRNAs were represented in the database). Only 10 tissues with the highest average miRNA expressions (rpmm normalized counts) are displayed for each miRNA changed in AD. E The top 10 most significantly enriched and neurodegeneration-related PANTHER knowledgebase forecast biological pathways based on targets of miRNAs found inside or outside the brain in modules E, F, and K. F Sankey diagram showing targets known to be involved in the pathogenesis of AD. MiRNAs with more than 3 related AD targets are displayed in the left panel. G Combined barplot of Vesiclepedia-based predicted proportions of miRNAs found in extracellular vesicles (EVs) and harboring secretion-promoting EXO motifs in WmiRNACNA clusters. Red frames indicate AD-related modules.