Fig. 2: Characterization of iECs in 2D culture.
From: miR126-mediated alteration of vascular integrity in Rett syndrome
a Immunostaining of ZO-1 (green) and F-actin (red) to quantify the tight junction properties. b Quantification of the ZO-1 area fraction rate. Mut-iECs displayed lower area fractions than WT-iECs. c Gene expression related to characterizing endothelial cells in HUVEC and WT-iEC and RTT-iEC. d Mut-iECs exhibited lower gene expression related to tight junctions (CLDN5, OCLN, and ZO-1), as measured by PCR (n = 3). e Measurement of TEER, indicating endothelial barrier functions. f, g Differential gene expression determined by RNAseq (n = 2). h GO enrichment analysis. Student’s t-test, **, p < 0.01, * p < 0.05.
