Fig. 3: Imaging-enhanced flow cytometry reveals heterogeneity of mutant endothelial cells.
From: miR126-mediated alteration of vascular integrity in Rett syndrome
a Schematic illustration of imaging-enhanced flow cytometry. b, c Single-cell gating and CD31-positive cells were determined. d Cell cycle analysis with DNA staining. e RTT-iECs had higher populations of the G0/G1 cycle. f Representative image of WT-iECs and Mut-iECs according to different cell cycles. Perimeter, pseudo-diameter, entropy, and circularity were quantified from the images. g UMAP plot shows the diverse population of endothelial cells. h, i Feature plot of ZO-1 indicates a unique cluster (Cluster 3, which expresses higher ZO-1). We determined the endothelial cell clusters (Clusters 2, 4, 5) and highly expressed ZO-1 cluster (Cluster 3). Clusters were determined based on four morphological features (perimeter, pseudo-diameter, entropy, and circularity). j Quantification of the number of cells in each cluster. The number of cells in Cluster 3 is higher in WT-iECs than in RTT-iECs.
