Fig. 5: Up-regulation of miRNA-126 in RTT-iECs negatively impacts vascular development and permeability.
From: miR126-mediated alteration of vascular integrity in Rett syndrome
a miR-126-3p was highly expressed in RTT-iECs compared with isogenic control endothelial cells. b miRDB predicts the target genes of miR126-3p. c Down-regulation of gene expression, targeted by miR126-3p. d miRNA-integrated analysis performed using RNAseq data and the predicted gene expression measured by PCR-based miRNA expression analysis, along with the miRDB prediction. e Down-regulation and up-regulation of the miRNAs from RTT-iECs over WT-iECs. f Seven up-regulated microRNA were determined by miRNA expression analysis, and e the related endothelial cells. g GO enrichment analysis (biological process) from the predicted down-regulated gene set determined by miRDB and miRNA expression analysis e. Significant down-regulated pathways (regulation of vascular development) were determined. h Cnetplot suggested that the genes involved in these signaling pathways include END1 and TEK1. i miRNA-RNA integrated analysis revealed 21 overlapping down-regulated genes, including EDN1 and TEK1, which are also consistent with the RNAseq analysis j, which potentially involved miR126-3p-mediated vascular alteration.
