Fig. 6: Knockdown of miRNA-126 rescues the phenotype and endothelial barrier functions in RTT-iECs.
From: miR126-mediated alteration of vascular integrity in Rett syndrome
a Lentivirus vector used to deliver antisense-miR126-3p and EGFP to down-regulate miR126 expression. b After endothelial differentiation, the cells were transfected with lentivirus, and EGFP-positive cells were sorted (n = 5). c, d Representative images and permeability analysis of RTT-iVascular MVNs with or without expressing miR126-3p knockdown constructs (green) perfused with 70 kDa Dextran (red) (n = 30). e RT-PCR revealed that EGFL7, TEK, EDN-1, and CLDN5 were down-regulated in RTT-iECs, and knockdown of miR126-3p recovered TEK and EDN-1 expression, along with the rescue of FOXO1 and CLDN5 (n = 3). f Gene function prediction involved in Ang/Tie2 signaling and tight junction stabilization based on the GeneMANIA algorithm. g Schematic illustration of the altered gene pathways in RTT-iECs. Student’s t-test, *, p < 0.05, **, p < 0.01, ****, p < 0.0001.
