Fig. 1

IL-22^−/− mice but not IL-22bp^−/− are highly susceptible to T.gondii-induced inflammation. a B6 mice were infected by gavage with 35 cysts of T.gondii (76 K strain) and IL-22 and IL-22 bp transcripts were measured by Q-PCR in the intestinal mucosa at indicated time point post infection (n = 4–5 mice for each time point). b–g B6, IL-22^−/− and IL-22bp^−/− mice were infected (I) or not (NI) by 35 cysts of T.gondii. b Survival was recorded (n = 10–12 mice per group). c–g Intestinal samples collected 7 days post infection from B6, IL-22^−/− and IL-22bp^−/− infected (I) mice or non-infected mice (NI) (n = 4–6 mice per group). c Relative parasite load was determined by Q-PCR. d Representative photographs of HE staining performed on 3 µm-thick paraffin embedded sections of intestinal tissue. Scale bar=200 µm. e Cell infiltration, exudate, edema, and epithelium destruction was evaluated to determine the histological score, f IFNγ, IL-1β, TIMP1, and KC levels were determined by ELISA, and g Reg3b, Reg3g, Il23a and Il17f transcripts by Q-PCR. Values are representative of two independent experiments expressed as mean ± SEM. *, **, and *** refer to P < 0.05, P < 0.01 and P < 0.001, respectively.