Fig. 6

Antigen-specific T-cell proliferation. PBMCs of healthy volunteers (n = 14) were collected on days 0 and 14 before receiving Dukoral vaccination, and at day 28. PBMCs were stimulated for 7 days with a cholera toxin B (10 μg/ml) or Dukoral (108 bacteria/ml) or b IMDM medium, ovalbumin (OVA) (10 μg/ml) or tetanus toxoid (TT) (5 lf/ml). Proliferating T cells (CD3+ Ki67+) were analyzed by flow cytometry. Data are shown as mean with SEM. Repeated measures ANOVA was performed per stimulation, no significant differences found. For the comparisons between stimulations and medium control per day a univariate ANOVA (GLM) was used, *p < 0.05; **p < 0.01; ***p < 0.001