Fig. 6
Assessment of the lung function. BALB/c mice were immunized with 2 × 108 particles of rAd-HA and rAd-NP plus increasing doses of rAd-IL-1β. Three days later, mice were anesthetized and the trachea canulated and connected to a ventilation device. Animals were exposed to increasing concentrations of vaporized methacholine. The baseline data were recorded without any vaporization, whereas “0” indicates the vaporization of the vehicle (PBS). Forced oscillation maneuvers were performed to measure the airway resistance RN (a), tissue damping G (b), and tissue elastance H (c). Depicted are the respective parameters plotted against the vaporized methacholine concentration. Data are represented as means + SEM and statistical significances were analyzed by one-way ANOVA followed by Tukey’s post test (n = 3–4; no statistically significant differences observed)
