Fig. 8
From: Modulation of bacterial metabolism by the microenvironment controls MAIT cell stimulation
Abundance of riboflavin-precursor antigens in E. coli is modulated by carbon sources and their concentration. a–d Relative abundance expressed as arbitrary units (AU) of indicated pyrimidines and ribityllumazines in extracts of E. coli grown in M9 medium supplemented by the indicated sole carbon sources. e–h Correlation of the metabolite levels with the E. coli Log10 ED50 of MAIT cell stimulation (as in Fig. 5e, f). a, e 5-A-RU (m/z 275.1004), b, f RL-6,7-diMe (m/z 325.1155), c, g 5-OE-RU (m/z 315.0949), and d, h 5-OP-RU (m/z 329.1105). In this type of analysis, 5-OP-RU cannot not be distinguished from reduced 6-hydroxymethyl-8-d-ribityllumazine (rRL-6-CH2OH,) as they have identical masses and fragment spectra.16,18,19 *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.001, analyzed using Kruskal–Wallis test with Dunnett’s multiple comparison test against values obtained with standard 40 mM glucose. Data show triplicates measured as technical replicas and are representative of two independent experiments
