Fig. 9
From: Modulation of bacterial metabolism by the microenvironment controls MAIT cell stimulation
Ex vivo MAIT cell responses are affected by E. coli growth conditions. t-SNE analysis performed on CD3+Vα7.2+CD161hi MAIT cells after PBMC stimulation in co-culture with A375.MR1 and E. coli extracts from the indicated growth conditions. Extracts were normalized to their OD600 (as in Fig. 5b, d). MAIT cells from one healthy donor were analyzed after down-sampling and concatenation of all conditions. Colors indicate cell expression levels of CD69 (upper panels) and PD-1 (lower panels). Experiments were performed twice with comparable results in two different donors
