Fig. 1
P. aeruginosa infection dynamically changes Regnase-1 expression in the lung. a, b Immunoblot image for indicated proteins in the lung at early phase (a) and latter phase (b) after intratracheal administration of heat-killed P. aeruginosa (1.0 × 107 cfu) to WT C57BL/6J mice. c Immunoblot image for Regnase-1 protein levels in the lung of WT C57BL/6J mice after intratracheal LPS administration (60 μg). d, e Immunoblot image for indicated proteins in the lung after intratracheal administration of heat-killed P. aeruginosa (1.0 × 107 cfu) to MyD88–/– mice (d) and Bcl10–/– mice (e). f RT-qPCR analysis of the indicated genes in WT C57BL/6J mice at the time points indicated after intratracheal administration of heat-killed P. aeruginosa (1.0 × 107 cfu) or PBS. Data are shown as the relative abundance compared with the average of PBS-treated mice (n = 3 for each group). g Immunoblot image for Ptgs2 proteins in the lung after intratracheal administration of heat-killed P. aeruginosa (1.0 × 107 cfu) to WT C57BL/6J mice. h IL-6 (left panel) or IL-12p40 (right panel) concentration in the lysate of lung from WT C57BL/6J mice at indicated time points after intratracheal administration of heat-killed P. aeruginosa (1.0 × 107 cfu). N.S. no stimulation. Images are the representative of two independent experiments (a–e, g). Data are shown as mean ± s.d. Two-tailed Student’s t-test was used for statistical analyses (f, h).
