Fig. 2
Loss of Regnase-1 in AECs protects against pulmonary P. aeruginosa infection. a, c, e Neutrophil populations in untreated lung (a), heat-killed P. aeruginosa (1.0 × 108 cfu) treated lung (48 h after treatment) (c), or lung and BALF at 24 h after intratracheal 1.0 × 105 cfu PA103 infection (e) harvested from control and AEC-Reg1-cKO mice. Images are the representative of n > 3. b Total neutrophil count in the lung from untreated AEC-Reg1-cKO (n = 5) or control (n = 9) mice. d Hematoxylin and eosin (H&E) staining of lung samples from control or AEC-Reg1-cKO mice 24 h after intratracheal PA103 (1.0 × 105 cfu) infection (n = 3). A cluster of inflammatory cells (thick arrow) and infiltration of neutrophils into the alveolar space (thin arrow) are indicated. f Bacterial load in the lung from control (n = 7) or AEC-Reg1-cKO (n = 5) mice 24 h after intratracheal infection of 1.0 × 105 cfu PA103. g, h Body weight changes (n = 10) (g) and survival rate (control: n = 34, AEC-Reg1-cKO: n = 23) (h) after intratracheal 1.0 × 105 cfu PA103 infection. i Survival rate after the re-infection with 1.0 × 106 cfu PA103 on the day 29 of primary infection with 1.0 × 105 cfu PA103 (control: n = 15, AEC-Reg1-cKO: n = 7). Data are shown as mean ± s.d. Two-tailed Student’s t-test (b, g), Mann–Whitnely U test (f), or Wilcoxon test (h, i) was used for statistical analyses
