Fig. 3
AEC Regnase-1 governs IgA secretion following P. aeruginosa infection. a Total IgA (left panel) or P. aeruginosa-specific IgA (right panel) concentration of BALF from control or AEC-Reg1-cKO mice at 22 days after intratracheal PA103 (1.0 × 105 cfu) infection. Each dot represents one sample. b Total IgA (left panel) or P. aeruginosa-specific IgA (right panel) concentration of serum from control (n = 9) or AEC-Reg1-cKO mice (n = 6) at 22 days after intratracheal PA103 (1.0 × 105 cfu) infection. Each dot represents one sample. c Flow cytometry analysis for the plasma cell populations of the lung from control or AEC-Reg1-cKO mice on the day 22 after PA103 infection. Images are representative of n = 5. d The rate of IgA+ plasma cells among plasma cells in the lung from control or AEC-Reg1-cKO mice on the day 22 after PA103 infection. Image is representative of n = 5. e The rate of IgA+ plasma cells among lymphocytes in the lung from control or AEC-Reg1-cKO mice on the day 22 after PA103 infection. f IgA concentration of BALF or serum and secretory IgA concentrations from WT and Regnase-1–/– (Reg1–/–) mice evaluated by ELISA. g Secretory IgA concentrations from WT and Reg1–/– mice evaluated by ELISA. Mann–Whitney U test (a, b, g) and two-tailed Student’s t-test (e, f) was used for statistical analyses
