Fig. 4

Genes associated with natural immunity and IgA secretion are upregulated in AECs lacking Regnase-1. a Scatterplot of gene expression profiles evaluated by RNA-sequencing for MTEC from WT or Reg1^–/– mice. The yellow and blue lines represent 10-fold and 3-fold induction, respectively. P-values were calculated by using DEseq2. b Summary for upregulated genes in MTEC derived from Reg1^–/– mice. Genes with RPKM <10 in WT-derived MTEC were excluded, and genes were ranked according to the ratio of gene expression levels between Reg1^–/–-derived and WT-derived MTEC. c RT-qPCR analysis of the indicated genes in WT or Regnase-1 knockout (KO) Calu-3 cell line (n = 3 for each genotype). Data are shown as mean ± s.d. Two-tailed Student’s t-test (c) was used for statistical analyses