Fig. 5
Loss of Regnase-1 enhances transcytosis and production of endogenous antibiotics. a Immunoblot for indicated protein levels in the lung of WT or Reg1–/– mice. Representative image of n = 3 for each genotype. b RT-qPCR analysis of the indicated genes in the lung from WT or Reg1–/– mice (n = 6 for each genotype). c Representative images of anti-IgA immunohistochemistry of lung samples from WT (n = 2) or Reg1–/– (n = 3) mice. d RT-qPCR analysis of the indicated genes in the lung from control or AEC-Reg1-cKO mice with intratracheal LPS (60 μg/head) treatment (control: n = 5, AEC-Reg1-cKO: n = 3). e RT-qPCR analysis for the indicated genes in the lung of WT C57BL/6J mice at 48 h after intratracheal administration of PBS or heat-killed P. aeruginosa (PA) (1.0 × 107 cfu/head) (n = 6 for each group). Data are shown as mean ± s.d. Two-tailed Student’s t-test was used for statistical analyses (b, d, e)
