Fig. 6
Regnase-1 in AECs governs the neutrophil accumulation. a Flow cytometry analysis for neutrophils in the lung from WT or Reg1–/– mice. Images are representative of three samples for each group. b Representative images of hematoxylin and eosin (H&E) staining of lung samples from WT (n = 4) and Reg1–/– (n = 6) mice. c, d RT-qPCR analysis of the indicated genes in the lung from control or AEC-Reg1-cKO mice without treatment (e, n = 6 for each group) or intratracheal LPS (60 μg/head) treatment (f, control: n = 5, AEC-Reg1-cKO: n = 3). e Representative flow cytometry analysis showing neutrophil populations or total neutrophil numbers in the lung (left) or BALF (right) of intratracheal LPS (60 μg/head)-treated AEC-Reg1-cKO or control mice for 48 h. Image is a representative or sum of n = 3 for each group. f Ratio of CXCR2-blocked and non-blocked neutrophils in the lung and BALF after the adaptive transfer of fluorescence-labeled CD45.1 WT neutrophils to Regnase-1–/– mice (n = 6). Data are shown as mean ± s.d. Two-tailed Student’s t-test was used for statistical analyses (c–f)
