Fig. 7

Epithelial and immune cell Regnase-1 coordinates optimal induction of acquired immunity. a RT-qPCR analysis of Ccl28 in the lung from WT and Reg1^–/– mice or control and AEC-Reg1-cKO mice (n = 6). b, c Plasma cells (b) or IgA⁺ plasma cells (c) populations in the lung from T-Reg1-cKO BM transferred control (n = 4) and AEC-Reg1-cKO (n = 5) mice. d Rate of plasma cells among lymphocytes in the lung of T-Reg1-cKO BM transferred control and AEC-Reg1-cKO mice. e RT-qPCR analysis of Ccl20 in the lung from control and AEC-Reg1-cKO mice (n = 6). f, g Representative flow cytometry image (f) and average ratio (g) of Th17 population among CD4⁺ T cells in the lung of T-Reg1-cKO BM transferred control (n = 4) or AEC-Reg1-cKO mice (n = 5). h Venn diagram for the numbers of commonly increased mRNAs in Regnase-1-deficient CD4⁺ T cells and AECs. i GOs enriched in Regnase-1-deficient CD4⁺ T cells and AECs. GOs commonly enriched (blue bars), specifically enriched in Regnase-1-deficient T cells (green bars) or AECs (red bars) are shown. Data are shown as mean ± s.d. Two-tailed Student’s t-test was used for statistical analyses (a, d, f, g)