Fig. 1
From: Epigallocatechin-3-gallate local pre-exposure application prevents SHIV rectal infection of macaques
EGCG inhibits viral infectivity of a broad spectrum of AIDS-related viruses. a TZM-bl cells were treated with the indicated concentrations of green tea-derived catechins (EC, EGC, ECG, and EGCG) for 10 min prior to infection with different strains of HIV-1 (Bal, NL4-3), SHIV (SF162P3N, KU-1), or SIV (mac239, mac251). Viral infectivity was assessed by luciferase activity, which is expressed as a percentage relative to that of the control (untreated). The half maximal inhibitory concentration (IC50) of EGCG is indicated, which was calculated based on the untreated control by the method of Reed and Muench. b Human peripheral blood monocyte-derived macrophages were incubated with the indicated doses of EGCG for 10 min prior to HIV-1Bal infection. Culture supernatant was collected on day 7 post-infection for HIV-1 reverse transcriptase (RT) assay. Cellular RNA was subjected to the real time RT-PCR for HIV-1 gag and GAPDH RNA. Data are expressed as HIV-1 RNA levels relative (%) to untreated control, which is defined as 100%. c Primary lymphocytes and macrophages from rhesus macaques were treated with or without EGCG (50 μM) for 10 min prior to SHIVSF162P3N infection. Intracellular gag RNA was measured by the real time PCR at day 5 post-infection. Data are shown as mean ± SD, representative of three independent experiments with 3–4 replicates. *P < 0.05, **P < 0.01 and ***P < 0.001
