Fig. 6
EGFR expression in intestinal epithelial cells mediates developmental effects of p40 in pups. Egfrfl/fl and Egfrfl/fl-Vil-Cre pups were treated with p40 as described in Fig. 2. a The fold change of bodyweight (a) and the percentage of bodyweight increase (b) at postnatal day 14 were calculated as described in Fig. 2. c Real-time PCR analysis was performed to detect Ki67 gene expression in the small intestine and the colon of 2-week-old mice. d, e The ileum tissues from 3-week-old mice were prepared for sucrose-isomaltase immunostaining and real-time PCR analysis to detect gene expression. f The colonic tissues from 3-week-old mice were prepared for ZO-1 immunostaining. g Feces from 2-week-old mice were collected for ELISA to detect fecal IgA levels. h Real-time PCR analysis was performed to detect APRIL gene expression in the small intestine of 2-week-old mice. In c, h the average of mRNA expression levels in the no-p40 group was set as 100%, and the mRNA expression level of each mouse was compared to the average. In a–c, e, and f–h, n = 5–9 in each group. In d, f, no-p40: n = 3–5; p40: n = 5–7 in each group
