Fig. 8 | Mucosal Immunology

Fig. 8

From: Supplementation of p40, a Lactobacillus rhamnosus GG-derived protein, in early life promotes epidermal growth factor receptor-dependent intestinal development and long-term health outcomes

Fig. 8

Neonatal p40 treatment augments innate immunity in adult mice. Mice were treated with p40 from postnatal day 2 to day 21, as described in Fig. 2. Colitis was induced in 6- to 7-week-old mice by TNBS and DSS, as described in Fig. 7. a, b The fecal IgA levels were examined using ELISA. *p < 0.01 compared to the no-p40 group with and without colitis. #p < 0.05 compared to the p40 group without TNBS treatment. c, d The colonic tissue sections were immunostained with MUC2 and counterstained with hematoxylin. The numbers of MUC2 positively stained cells are shown. e mRNA was isolated from the colonic tissues for real-time PCR analysis of Muc2 mRNA expression levels. The average of mRNA expression levels in the control mice in the no-p40 group was set as 100%, and the mRNA expression level of each mouse was compared to this average. n = 5–7 in each group. In d, e, *p < 0.05 compared to control mice in the no-p40 group. #p < 0.05 compared to the p40 group with DSS treatment

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