Fig. 2
From: C3a receptor antagonism as a novel therapeutic target for chronic rhinosinusitis
Sinonasal tissues from patients with CRSwNP have heightened levels of C3 and C3a. a Single-cell suspensions were prepared by mechanical separation and stained for the epithelial cell marker EpCAM. Following permeabilization cells were immunostained for C3 and C3a. Note that human sinonasal epithelial cells were the predominate cell type staining positively for C3 and C3a. b Quantification of the percent of viable cells double positive for EpCam and C3 or C3a. Note the significant increases seen in EpCam + C3 and C3a expression sinonasal epithelial cells in patients with CRSwNP. n = 4 per group. Statistics shown are unpaired Student's t test between indicated groups. *p < 0.05. c Complement mRNA levels were measured in control and CRSwNP HSNECs by qRT-PCR. Relative HSNEC expression of C3, C4, C5, and fB was assessed and expressed as a fold change in CRSwNP as compared to control HSNEC expression. Note the increase in C3, C4, C5, fB, and fH. No gene expression for C1 or C9 could be detected in HSNECs from either group (data not shown). n = 5–8 per group
