Fig. 5 | Mucosal Immunology

Fig. 5

From: Synergistic activation of pro-inflammatory type-2 CD8+ T lymphocytes by lipid mediators in severe eosinophilic asthma

Fig. 5

PGD2 and LTE4 promote type-2 cytokine production in cultured Tc2 cells. a, b mRNA levels by qPCR in Tc2 cells (a) and protein levels by ELISA in the cell supernatants (b) for IL-5 and IL-13 after treatment with various concentration of PGD2 and LTE4. c Half maximal effective concentration (EC50) of PGD2 or LTE4 for IL-13 production in Tc2 cells compared with that in Th2 cells. d, e mRNA levels in Tc2 cells measured by qPCR (d) and protein levels in the cell supernatants measured by Luminex (e) for type-2 cytokines after treatment with PGD2 and LTE4 alone or their combination. The mRNA levels in control samples were treated as onefold. f Increase of IL5- and IL13-mRNA-positive Tc2 cells after treatment with PGD2 and LTE4 alone or their combination detected by using PrimeFlow RNA assay. *p < 0.05, **p < 0.005, ***p < 0.001 (n = 3 for a–c and f; n = 6 for d and e)

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