Fig. 3

Gene expression and cytokine profile of TRAG colitis. (a) Principal component analysis of gene expression in TRAG vs. RAG1^−/− colonic mucosa. (b, c) Molecular drivers of the differential gene expressions patterns identified using Ingenuity Pathway Analysis of genes associated with colitis in TRAG mice implicates the (b) ILC and (c) JAK1/2 pathway. (d) Quantitative PCR showing increased expression of INFγ, IL-22, and IL17, but not TNF, in the mucosa of TRAG mice. (e) Cytokine secretion by LPLs isolated from RAG1^−/− vs. TRAG mucosa showing increased secretion of IFNγ, TNF, IL-5, IL-9, IL-22, and IL-1β from LPLs of TRAG mice. (f) Cytokines detected but not statistically different between TRAG and RAG1^−/− LPLs included IL-17F, IL-17A, GM-CSF, IL-23, IL-4, IL-12, and IL-13. (Mann–Whitney) *p < 0.05, **p < 0.01, ***p < 0.001 (n = 7–10 mice/group (except TRAG array group where n = 4))