Fig. 4
From: Dynamics of helper CD4 T cells during acute and stable allergic asthma
Activation of non-classical transcriptional pathways and related cytokines during acute allergic asthma. a Heat map (blue to red) of normalized fold change (vs. the healthy control group) of median cytokine concentration secreted by helper T cells after TCR stimulation with a-CD3/CD28 microbeads. Sorted Th1, Th2, Th17 and TFH cell populations were stimulated and incubated (37āĀ°C; 5% CO2) for 48āh. Supernatants were collected and examined for expression of cytokines in Th1, Th2, Th17, and TFH cells using Luminex assay. b Heat map of normalized fold change of transcriptional factors of helper CD4 T cells during asthma. Poly-clonally stimulated sorted Th1, Th2, Th17, and TFH cells were lysed and analyzed for their transcription factor expression by RT-PCR. Fold change of each transcription factor expression in acute or stable asthma was calculated versus its corresponding expression in CD4 T cell subsets from control donors
